To investigate the function of FPN1 in ECs on neurological injury in mice with acute cerebral ischemia, we first examined the cerebral infarct volume and evaluated the effects of neurological injury, in terms of body weight, mNSS, adhesive removal, and gait analysise, in the cerebral ischemia mice with or without conditional disruption of Fpn1. One day after the operation, i.e., the acute stage of ischemic stroke, Fpn1 knockout in ECs resulted in reduced cerebral infarct volume, decreased mNSS, shortened contact and removal times of adhesion, and lessened neurological function impairments. Here, SLC40A1 is linked to cerebral infarction.