We evaluated the effects of DS-1594a·HCl and DS-1594a·succinate on differentiation and population of leukemia-initiating cell (LIC) fractions in MLL1-r AML-like cells, using RT‒qPCR, MGG staining, FCM, serial colony formation and growth assays. The gene discussed is KMT2A; the disease is acute myeloid leukemia.