First, the AdV vector was characterized in vitro, confirming that the CRISPR/Cas9 system could be successfully transduced into various cancer cells (Hepa 1‐6, SMMC‐7721, C3A, SK) by virtue of the high invasiveness of Ad5, which is superior to that reported by non‐viral delivery vehicles for PD‐L1 editing, such as cationic gold nanorods and polyethylenimine derivatives.[15, 16]. Here, CD274 is linked to cancer.