This study aimed to investigate the role of syringin in improving heart function during myocardial ischemia/reperfusion (I/R) and to determine whether the sirtuin 1/peroxisome proliferator‐activated receptor gamma coactivator 1 alpha (SIRT1/PGC‐1α) pathway contributes to this cardioprotective effect in vivo and in vitro. This evidence concerns the gene SIRT1 and myocardial ischemia.