Cloning of the Apo-A1 gene into the downstream sequence of the CD63 in donor cells produced exosomes that expressed Apo-A1 on the surface of bilayer lipid membranes, and these exosomes were specifically recognized by scavenger receptor class B1 positive hepatocellular carcinoma cells, thereby enhancing the tumor targeting of the exosomes. The gene discussed is APOA1; the disease is hepatocellular carcinoma.