We have demonstrated that encapsulated tumor antigens (Tag) made up of purified membrane proteins from murine 4T1 tumor cells to generate immune-activating nanoparticles (CpG-NP-Tag) reduced primary tumor size and tumor weight associated with an accumulation of CD4+ and CD8+ T cells as well as the detection of IFN-γ within the solid tumor mass [17]. This evidence concerns the gene CD4 and neoplasm.