In this pathway, we found that the upregulation of the SLC1A5 gene, which encodes a glutamine transporter protein on the cell membrane surface, promoted the production of 2-Oxoglutarate from glutamine by colon cancer cells to provide a constant source of energy for cell proliferation via the TCA cycle, whereas the involvement of glutamate in proline metabolism in mitochondria promoted the significant upregulation of proline as a metabolite. This evidence concerns the gene SLC1A5 and malignant colon neoplasm.