LYZ and infection: In this study, we investigated the involvement of Spy1094 and Spy1370 on S. pyogenes virulence by generating S. pyogenes gene deletion mutants and Lactococcus lactis gain-of-function mutants and analysed them for lysozyme sensitivity, antimicrobial resistance, biofilm formation, survival in human whole blood and in-vivo virulence using a Galleria mellonella (greater wax moth) larvae infection model.