In men with NAFLD, the PNPLA3 variant was associated with increased ALT, the TM6SF2 variant with decreased TAGs, the GCKR variant with increased TAGs, the PPP1R3B variant with decreased HDLC and decreased FFAs, and the HSD17B13 variant with decreased ALT and TAGs. Here, PNPLA3 is linked to metabolic dysfunction-associated steatotic liver disease.