First, we investigated the effects of the six genetic variants (PNPLA3 rs738409-G, TM6SF2 rs58542926-T, MBOAT7 rs641738-T, GCKR rs780094-T, PPP1R3B rs4841132-A, HSD17B13 rs72613567:TA) on metabolites in men without NAFLD (Table 2). This evidence concerns the gene PNPLA3 and metabolic dysfunction-associated steatotic liver disease.