In men with NAFLD, we found novel statistically significant positive associations of the PPP1R3B variant with nine metabolites, including two lyso-phosphatidylethanolamines (lyso-PEs) and four steroids, and novel statistically significant inverse associations with two metabolites belonging to the tryptophan pathway, N-acetylkynurenine [2] (beta −0.098, p = 2.4−6) and xanthurenate (beta −0.085, p = 3.6 × 10−5) (Supplementary Table S2). This evidence concerns the gene PPP1R3B and metabolic dysfunction-associated steatotic liver disease.