Combined acteoside and 5- fluorouracil (5-FU) treatment led to the arrest of the G1 phase, enhanced apoptosis by altering the Bax, Bcl-2, and p53 protein levels and gene expression, and reduced the PI3K and p-AKT/total AKT ratio in colorectal cancer cell lines Caco-2 and HCT-116. This evidence concerns the gene BCL2 and colorectal cancer.