At the time, pyrosequencing and the first models of allele-specific real-time PCRs (sensitivity 7–10%), requiring less tumor tissue DNA and shorter hands-on time procedures to achieve KRAS and NRAS mutations in codons 12, 13, 59, 61, 117, and 146, represented the most commonly used marketed techniques for colorectal cancer [10]. This evidence concerns the gene KRAS and colorectal cancer.