This present study sought to evaluate the expression of hepatic nuclear factors (HNF1α, HNF4α, and LXRb) and miRNAs (HNF4α specific: miR-194-5p and miR-122*_1) that regulate CYP7A1 transcription in HIV-positive Black South African women on cART and presenting with gallstones relative to HIV-negative patients with gallstone disease. This evidence concerns the gene HNF1A and gallstones.