CHAC1 and glioblastoma: At the transcriptional level, we found 2 genes, NCOA4 and ALOX5, had significant differences in expression between GBM and normal brain tissues by qRT-PCR, while 4 genes, CHAC1, CRYAB3, ZEB1, and STEAP3, changed in the same direction as the public dataset, but did not reach statistical significance.