TGFB1 and glaucoma: However, since these concepts remain speculative at present, and currently used immortalized TM cells do not always behave the same as primary HTM cells, additional investigations using additional methodology such as RNA sequencing as well as the inhibition of specific candidate molecules by SiRNA and others in addition to using the same concentrations of TGF-β isoforms and primary HTM cells obtained from several glaucoma and non-glaucoma individuals will be required.