In 47 patients with bladder cancer, benign perivesical nodes were examined for tenascin-C expression, an extracellular matrix glycoprotein downregulated in healthy tissues but expressed in tissue remodeling, showing that its expression was higher in LNs from patients that harbored metastasis in other LNs (N+), than in patients with no LN metastasis (N0), suggesting that this factor is a specific feature of the lymph node pre-metastatic niche [7]. This evidence concerns the gene TNC and urinary bladder cancer.