TP53 and osteosarcoma: In contrast to the Hsp40-inhibiting chalcone 29, the trans-chalcone 35a (50 μM) and its 5-fluoro-2-hydroxy derivative 35b (10 μM) activated Hsp40 and p53 expression in U2OS osteosarcoma cells, accompanied by suppression of CRM1 (Figure 5) [134].