The quinoline analog 12 (Figure 3) of compound 1 was slightly more active against and selective for Grp78 (KD = 0.6 μM for Grp78, 0.3 μM for Hsp70) than compound 1 (KD = 0.8 μM for Grp78, 0.1 μM for Hsp70), however, compound 12 showed no antiproliferative activity against HCT-116 colon cancer cells in contrast to compound 1 (GI50 = 5.0 μM) [70]. This evidence concerns the gene HSPA1A and colonic neoplasm.