PPARGC1A and Hyperinsulinemia: When hsa-miR-193b-3p was overexpressed in HepG2 cells for 72 h using a mimic, we observed robust downregulation of PPARGC1A mRNA expression at basal glucose concentration (5 mM glucose, Figure 1A), under hyperglycemia (20 mM glucose, Figure 1B), under hyperinsulinemia (5 mM glucose and final 24 h 10 nM insulin, Figure 1C) and under hyperglycemia/hyperinsulinemia (20 mM glucose and final 24 h 10 nM insulin, Figure 1D).