Among the 31 RNA transcripts validated, the expression of COL11A1, MUC12, FRMD5, FCGBP, EGFL6, FN1, KLK5, ITGA9, DCX, WNT4, WNT2, WNT16, WIF1, CXCL13, CBX8, NDC80, NTM, PPP1R14C, BMP7, EZH2, IGFBPL1, MTMR8, ATP5MC1P1, CYP19A1, HMX1, CACNA1D, and RGS4 was significantly higher, while the expression of S100A1, RAB37, and S100A4 was significantly lower in leiomyomas compared to matched myometrium (Figure 5A–D). This evidence concerns the gene CBX8 and leiomyoma.