The perturbation versus over-representation pathway analysis showed that the most significant pathways in AA-SScL fibroblasts were “Calcium signaling,” “Regulation of actin Cytoskeleton,” “Gap junction,” “Prostate cancer,” “Choline metabolism in cancer,” “Melanoma,” “Taste transduction,” “Intestinal immune network of IgA production,” “Alcoholism,” and “Steroid biosynthesis” (Figure 5C). The gene discussed is CD79A; the disease is alcohol dependence.