Considering the role played by the endocannabinoid system in prostate cancer, and the potential anticancer effect of DIM, the aim of this study was to characterize the ability of DIM to interact with both CB1 and CB2 receptors and to evaluate any effect on apoptotic processes in cellular models of prostate cancer, such as PC3 (androgen-independent human prostate cancer cells) and LNCaP (androgen-dependent human prostate cancer cell line) that naturally express the endocannabinoid system [29,30]. Here, CNR1 is linked to prostate carcinoma.