The analysis of two additional RNA sequencing datasets obtained from different types of ALS samples (FUSH517Q in vitro derived MNs and ALS post-mortem tissues) allows to conclude that the biogenesis of the circRNAs characterized in this study is specifically affected by P525L FUS mutation, since they do not vary or do not show the same trend of variation in the other two conditions. The gene discussed is FUS; the disease is amyotrophic lateral sclerosis.