When HCC patients were further sub-classified based on AFP detection (cutoff = 20 ng/mL) into AFP-negative HCC patients (n = 12/39) and AFP-positive HCC patients (n = 27/39), significant downregulation of plasma hsa-miR-21-5p levels in AFP-negative HCC patients (p = 0.039) was evident, as presented in Table 5. The gene discussed is AFP; the disease is hepatocellular carcinoma.