The infusion of PRL subcutaneously in female mice resulted in hyperprolactinemia (with a mean infusion of 260 ng/mL) which was then associated with a loss of estrous cyclicity, anovulation (as reflected by a decrease in ovarian corpora lutea), a reduction in circulating luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels, and a decrease in pituitary luteinizing hormone (Lhb) and follicle-stimulating hormone (Fshb) mRNA [86]. This evidence concerns the gene PRL and hyperprolactinemia.