In this study, hsa_circ_0004214 is used to assist immunohistochemical diagnosis of glioma grading, but its specific quantitative relationship with GFAP and Ki67 needs to be further explored in the future, and the underlying mechanisms of how hsa_circ_0004214 regulates Ki67 and GFAP expression and cell proliferation were unclear. The gene discussed is MKI67; the disease is central nervous system cancer.