Notably, the MDA PCa 2b cells also demonstrated the highest phosphorylation states of the AKT, mTOR, and S6 (pAKT-T308, pAKT-S473, pmTOR, and pS6), suggesting that the AKT/mTOR signaling is significantly upregulated in metastatic AA PCa (MDA PCa 2b, with highest PIK3CD-S/PIK3CD-L ratio) vs. metastatic EA PCa (22Rv1, LNCaP, and PC-3) and AA primary PCa RC77 T/E (Figure 1D). Here, AKT1 is linked to posterior cortical atrophy.