To investigate whether the TLLSs associating with CD169+ TAMs in PTs were functional tertiary lymphoid follicles, and to confirm the association between CD169+ TAMs and TLLSs in breast cancer, gene signatures of mature tertiary lymphoid structure (TLS) [32] from bulk RNAseq from 8164 patients of the SCAN-B cohort were investigated, allowing analysis in a larger, contemporary and representative cohort. This evidence concerns the gene SIGLEC1 and breast cancer.