We observed a subset of N-glycans in different parts of the VX2 tumor tissue after the PNGase F treatment (Figure 3) with five glycans that were identified based on accurate mass measurements, all as sodium-cationized ions: Hex7HexNAc2 (m/z 1581.52), Hex3dHex1HeNAc4 (m/z 1485.49), Hex6dHex1HexNAc3 (m/z 1769.06), Hex5dHex1HexNAc4 (m/z 1809.59), and Hex6HexNAc5 (m/z 2028.19). The gene discussed is NGLY1; the disease is neoplasm.