Figure 2J–M reveal that EUG 500 μM significantly increased the level of caspase-3 in HCT-116 colon cancer cells. The mean percentages of caspase-3 increased from 30.3% in the DMSO (control) group to 89.4% in EUG (500 μM) treated cells with a p-value of <0.0001 (N = 4, mean ± standard deviation). Figure 2 indicates that the controlled release of a high dose of EUG is necessary for successful irreversible apoptosis in CRC cells. Here, CASP3 is linked to malignant colon neoplasm.