We used tumor tissue derived from the tails of Tg(Grm1)EPv mice, a melanoma mouse model derived from C57BL/6 mice that carries a metabotropic glutamate receptor 1 (Grm1) transgene under the control of the dopachrome tautomerase promoter, resulting in melanocyte-specific overexpression [51]. Here, GRM1 is linked to melanoma.