Pyroptosis was triggered by DOX at concentrations ranging from 0.5 to 5 μmol/L in melanoma cell lines SK-MEL-5, SK-MEL-28, and A-375 that had high levels of deafness, autosomal dominant 5, isoform CRA a (DFNA5) expression, but not in the human breast cancer cell line MCF-7, which had low levels of DFNA5 expression. The gene discussed is GSDME; the disease is breast cancer.