TRIM32 deficient myoblasts exhibited an increased level of PIAS4 together with global SUMOylation and other replicative senescence mediators, such as heterochromatin protein 1 (HP1γ) and p53, which are all typical features found in sarcopenia and type II fiber atrophy associated with myopathy and LGMD2H. Here, TRIM32 is linked to autosomal recessive limb-girdle muscular dystrophy type 2H.