Applied to three CML-derived iPSCs, we show that this technology can be used to mimic cytological and genomic instability patterns observed in primary blast crisis cells, including the overexpression of IL2RA/CD25, that have been validated in a cohort of primary CML patients in AP/BC-CML. This evidence concerns the gene IL2RA and chronic myelogenous leukemia, BCR-ABL1 positive.