On the other hand, the results of the bioinformatics analysis showed binding sites for bHLH and Sp1 transcription factors, as well as a PPI network, where the TBX20 protein interacts with proteins encoded by cardiac genes, therefore, exhibiting an important role in the biological processes involved in the heart formation and gene-associated diseases such as ventricular and septal defects. The gene discussed is TBX20; the disease is Abnormal cardiac septum morphology.