Based on the binding ability between RUNX1 and p300, we previously found that RUNX1 facilitated p53-mediated transcriptional activity via a complex formation between p53 and p300 in p53-proficient osteosarcoma U2OS cells and HCT116 colon cancer cells treated with adriamycin (ADR) [55]. This evidence concerns the gene EP300 and osteosarcoma.