By using PE3 and a silent mutation in the PAM (to avoid a second prime editing event on an already edited allele), they introduced the A > G substitution (at +7 position in the pegRNA) in 20% of the liver organoid clones in the ABCB11 gene that causes bile salt export pump deficiency. The gene discussed is ABCB11; the disease is hyperinsulinemic hypoglycemia, familial, 4.