MMR dependent autophagy was further validated in another MSH6 deficient cell line, DLD-1colorectal adenocarcinoma cells, where no autophagy induction was observed upon TMZ treatment using MDC staining; conversely, re-expression of MSH6 restored the ability of DLD-1 cells to undergo autophagy after TMZ treatment. This evidence concerns the gene MSH6 and adenocarcinoma.