In our experiment, H522, H522(CD19+), PC-3M, PC-3M(CD19+), A431, A431(CD19+), MDA-MB-231, and MDA-MB-231(CD19+) tumor cell lines were seeded at 5 × 104 cells per well in an E-plate, and after 24 h 1 × 105 CAR-T cells were added per well. Here, CD19 is linked to neoplasm.