Multiplex analysis showed an increase in the levels of some cytokines/chemokines upon incubation of CAR-T and tumor cells—GM-CSF, I-309, MIF, and TNFα for H522(Kat+CD19+) cells; IL-4 and MIF for PC-3M(Kat+CD19+) cells; IFNγ, I309, IL-2, IL-8, MIG, MIP1α, and TNFα for A431(Kat+CD19+) cells; and TNFα, IL-2, IL-6, MIG, and GM-CSF for MDA-MB-231(Kat+CD19+) cells. The gene discussed is TNF; the disease is neoplasm.