CAR-T cells were able to penetrate three-dimensional tumor-like structures formed by H522(Kat+) and H522(Kat+CD19+), PC-3M(Kat+) and PC-3M(Kat+CD19+), A431(Kat+) and A431(Kat+CD19+), and MDA-MB-231(Kat+) and MDA-MB-231(Kat+CD19+). The gene discussed is CD19; the disease is neoplasm.