For example, TriMix, a construct containing immunostimulatory mRNAs encoding for CD40 ligand, CD70 and TLR4, if associated to mRNA vaccines leads to a shift from the generation of Treg cells to the generation of Th-1 cells [39] and to an increase of anti-tumor activity of anti-melanoma mRNA vaccine in preclinical and clinical studies [40,41]. This evidence concerns the gene TLR4 and melanoma.