The two SwR-STEAP1 CAR T cell variants were harvested after 21 days and co-cultured with STEAP1+/− tumor cells (wild-type 22Rv1 and 22Rv1-KO), as well as with 22Rv1 cells overexpressing TGFβ (22Rv1-TGFβ) or supplemented with rhTGFβ (22Rv1+rhTGFβ). The gene discussed is TGFB1; the disease is neoplasm.