In order to investigate whether anti-titin antibodies differ between MG subgroups, we then compared the titin binding pattern of sera from patients with hyperplastic thymus (most of early onset MG, <40 years old), with involuted thymus (the majority were of late onset MG, >40 years old), or with thymoma (the majority were of early onset MG). This evidence concerns the gene TTN and myasthenia gravis.