Utilizing immunohistochemistry and digital image analysis, we found increased MANF expression in the ducts of chronic pancreatitis relative to normal tissue, a correlation between MANF ductal expression and the amount of fibrosis present, and reduced MANF expression in the acini of chronic alcoholic pancreatitis relative to non-alcoholic pancreatitis and the normal pancreas. Here, MANF is linked to chronic pancreatitis.