Focusing on the analysis of the prostate cancer pathway, genes upregulated (GRB2, MAPK1, PIK3CG and PIK3R5; black color) and downregulated (AKT3, CCND1, CHUK, CREBBP, GSK3β, LEF1, MDM2, RB1 and SIN3A; gray color) in EV-mRNA from an mPC plasma sample compared to EV-mRNA from BPH plasma sample are illustrated in Figure 6B. The gene discussed is LEF1; the disease is prostate cancer.