In order to analyze the sensitivity in the detection of mutations in the EV-DNA of EVs isolated with ExoGAG, a spiking of 3 mL of control plasma sample with 1.29 × 106, 6.45 × 105 and 1.29 × 105 EVs from the colorectal HCT116 cell line isolated by ultracentrifugation was performed; this was also carried out in order to detect the PIK3CA H1047R mutation present in this human colorectal cancer cell line [39]. The gene discussed is PIK3CA; the disease is colorectal cancer.