TriMix, a mixture of monocyte-derived dendritic cells electroporated with mRNA encoding CD70, CD40 ligand, and constitutively active TLR4, as well as the tumor-associated antigens tyrosinase, gp100, MAGE-A3, or MAGE-C2 were administered together with ipilimumab in patients with advanced melanoma [206]. This evidence concerns the gene CD40LG and melanoma.