TP53 and familial pancreatic carcinoma: To further prove the necessity of mut K-Ras in activating the modified p14ARF promoter, we transiently transfected Ap1e-E2Fe-p14ARF-TATA-Luc (p14ARFmin-Luc) constructs and control plasmids into the human pancreatic cancer line BxPC-3 cells (wt K-Ras/mut p53) and PANC-1 cells (mut Ras/mut p53).