Taken together, a singular exposure to TSLP/SEB is sufficient to drive PBMCs to produce AD biomarkers like TARC, known to correlate with disease severity, as well as master Th2 cytokines, without the contribution of epithelial cells nor exogenous cytokines, such as IL-4 or IL-2, nor artefactual Th1-blocking antibodies, as used in traditional in vitro Th2 models. Here, SETBP1 is linked to Alzheimer disease.