To identify novel function mutations, we carried out the target sequencing of the C9orf9 coding region in a validation group of 50 pairs of CRC samples and detected a novel splicing mutation (ISV2 + 1G > A) in the Ex-6 patient and a missense mutation (c.25C > T) in the Ex-36 patient (Table 1). This evidence concerns the gene SPACA9 and colorectal carcinoma.