The NRF2 activation of the STCs was elucidated by determining the NQO1 induction (Prochaska microtiter plate assay) and expression of representative antioxidant genes (HMOX1, NQO1, GCLC, GCLM, TXNRD1, PRDX1, GPX2, GSTP1, GSTM2, GSTA1, and G6PDX by qPCR) in mouse hepatoma (Hepa-1c1c7) cells. The gene discussed is GCLM; the disease is hepatocellular carcinoma.