Histological analysis of H&E-stained liver sections revealed the pathological changes of hepatic steatosis in the livers of CHIP+/− mice fed HF–HS diet (Figure 4f, top), and Oil Red O staining showed significantly more lipid accumulation in the livers of CHIP+/− mice fed HF–HS diet than CHIP+/+ mice fed HF–HS diet (Figure 4f, bottom). The gene discussed is STUB1; the disease is Hepatic steatosis.