Neurodegeneration in AD-Neuro cells was studied by gene analysis which showed significant downregulation in the expression of the neuronal markers synapsin1, tuj1, neurofilament and neuropilin (Figure 3b) and a decrease in expression of functional cholinergic markers such as ChAT and α7nAChR (Figure 3c). The gene discussed is CHRNA7; the disease is Alzheimer disease.