Next, upon investigating the effect of AD201 inhibition on Wnt-related genes, we observed higher expression of Axin, APC, β-catenin and Dvl1, whereas there was a decrease in GSK-3β and sFRP4 expression in AD-Neuro cells with AD201 inhibition (Figure 7b(i)), indicating that Wnt-canonical signaling was expectedly activated upon downregulation of AD201. This evidence concerns the gene SFRP4 and Alzheimer disease.