The known VISTA ligands are V-set and Ig domain-containing 3 (VSIG3, expressed on colorectal cancers, hepatocellular carcinomas, and intestinal-type gastric cancers) and, at an acidic pH, such as in the TME, P-selectin glycoprotein ligand 1 (PSGL-1). The gene discussed is SELPLG; the disease is hepatocellular carcinoma.